FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16536517
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG874250.5286784393593886TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT122350.060.11303342
TCGTATG131150.058.7363843
TATGCCG132300.058.12222346
TATCTCG129450.056.7112839
ACGTCTG164500.054.7583115
CGTATGC141550.054.46962444
GTATGCC144050.053.7160545
CACCGAT148900.053.5901231
CAGTCAC155200.053.1919127
ATGCCGT146300.052.56028747
CACGTCT173250.052.09167514
TGTATCT145850.050.90225637
ACACGTC178900.050.42647613
CGATGTA158300.049.9509934
AGTCACC163400.049.931728
GTATCTC148250.049.61279338
GCCGTCT155400.048.7949949
CCGTCTT156500.048.5400550
GTCTGAA185950.048.51609817
CCAGTCA177400.047.66303626
Produced by FastQC (version 0.11.2)