FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12280522
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG441010.3591133992512696TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT63850.057.59609242
ACTTAGG70900.057.076532
TCGTATG69450.056.4292943
TATGCCG71800.054.91937646
ACGTCTG90100.052.68669515
GTCACTT79650.051.75921629
AGTCACT81100.051.68437228
GTATGCC78150.050.5006945
CACGTCT96250.049.5350814
CAGTCAC86350.049.42075727
TTAGGCA81500.049.14569534
ACACGTC97800.048.6085213
ATGCCGT82500.047.71286847
CGTATGC82800.047.49774644
GTCTGAA104250.045.6018317
CCAGTCA101400.043.95667626
CTTAGGC91900.043.7343933
CACACGT109100.043.4785112
TCTCGTA85550.042.90620441
CCGTCTT92400.042.264450
Produced by FastQC (version 0.11.2)