FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14042038
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG451390.321456187485036TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65950.055.9742
TCGTATG78500.055.28392443
TATGCCG78700.054.79295746
ACGTCTG110700.053.38633715
AGTCACT87900.052.2783428
ACTGACC83700.052.01622432
GTATGCC87450.049.50776745
CACGTCT119250.049.49989314
CAGTCAC98200.049.28894827
ACACGTC121400.048.96322313
CGTATGC90500.048.2202944
GTCTGAA130050.045.86749317
CACACGT130100.045.661812
ATGCCGT95550.045.23866747
TCCAGTC134000.044.20474225
CCAGTCA118950.043.6492726
CTGACCA101350.043.1962333
GTCACTG108000.042.0702129
CTCCAGT142300.041.89322324
TCTGAAC142350.041.88014618
Produced by FastQC (version 0.11.2)