FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15210145
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG371290.24410681160501757TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG62650.054.6236243
CTCGTAT56650.053.89310542
TATGCCG66300.051.14830846
ACGTCTG86600.049.16026715
CGTATGC69950.048.77512744
AGTCACA78850.045.94053328
CAGTCAC81250.045.09289627
CACGTCT97400.043.9920214
GTATGCC78050.043.66920545
ATGCCGT79600.042.64550447
ACACGTC102050.042.0539913
GTCACAT86600.041.07231529
TCACGAT89300.039.6365435
GTCTGAA110350.038.8925517
ATCACGA93050.038.81785634
CCAGTCA100950.038.58287426
CACACGT115700.037.12201712
TCCAGTC116100.036.60867725
TCTCGTA84900.036.04186241
CACGATC85750.035.6851836
Produced by FastQC (version 0.11.2)