FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12549201
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG466570.3717925946042302TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT70200.058.87162842
TCGTATG75200.057.57280743
TATGCCG76600.056.83627346
CGTATGC84750.051.2887844
GTATGCC85900.050.76298545
ACGTCTG103650.049.92745615
ATGCCGT87600.049.4630147
CAGTCAC97100.048.03632727
CACGTCT110750.046.7882514
ACACGTC114250.045.5351813
AGTCACC103850.044.54865328
CCGTCTT96300.044.4933950
CCAGTCA108350.044.22646326
GTCTGAA117300.044.147617
TCTCGTA96200.043.35535441
TCCAGTC120600.042.4515625
GCCGTCT101050.042.29966449
CACACGT123800.041.9384712
CTCCAGT125500.041.04133224
TCTGAAC131150.039.74848618
Produced by FastQC (version 0.11.2)