FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13458380
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1018800.7570004710819579TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT134900.061.29703542
TCGTATG138800.061.1653643
TATGCCG141300.059.9864146
TATCTCG139700.059.316839
GTCACGC148600.058.08618529
AGTCACG150350.057.66206428
GTATGCC149450.056.85280245
ACGTCTG163900.056.7256315
CGTATGC150200.056.66078644
CGCCAAT152300.056.40362533
CAGTCAC156500.056.0131327
GCCAATA155000.055.42111234
ATGCCGT155800.054.47001347
TCACGCC158750.054.35019330
CACGCCA160050.053.99495731
ATATCTC154300.053.92797538
CCAATAT158950.053.8274235
ACACGTC175200.053.3814213
CACGTCT174550.053.3434314
ACGCCAA165050.052.37994832