Basic Statistics
Measure | Value |
---|---|
Filename | HFNNGBGX2_n01_4s-52.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13458380 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 101880 | 0.7570004710819579 | TruSeq Adapter, Index 6 (100% over 63bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 13490 | 0.0 | 61.297035 | 42 |
TCGTATG | 13880 | 0.0 | 61.16536 | 43 |
TATGCCG | 14130 | 0.0 | 59.98641 | 46 |
TATCTCG | 13970 | 0.0 | 59.3168 | 39 |
GTCACGC | 14860 | 0.0 | 58.086185 | 29 |
AGTCACG | 15035 | 0.0 | 57.662064 | 28 |
GTATGCC | 14945 | 0.0 | 56.852802 | 45 |
ACGTCTG | 16390 | 0.0 | 56.72563 | 15 |
CGTATGC | 15020 | 0.0 | 56.660786 | 44 |
CGCCAAT | 15230 | 0.0 | 56.403625 | 33 |
CAGTCAC | 15650 | 0.0 | 56.01313 | 27 |
GCCAATA | 15500 | 0.0 | 55.421112 | 34 |
ATGCCGT | 15580 | 0.0 | 54.470013 | 47 |
TCACGCC | 15875 | 0.0 | 54.350193 | 30 |
CACGCCA | 16005 | 0.0 | 53.994957 | 31 |
ATATCTC | 15430 | 0.0 | 53.927975 | 38 |
CCAATAT | 15895 | 0.0 | 53.82742 | 35 |
ACACGTC | 17520 | 0.0 | 53.38142 | 13 |
CACGTCT | 17455 | 0.0 | 53.34343 | 14 |
ACGCCAA | 16505 | 0.0 | 52.379948 | 32 |