FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14197974
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG313460.22077797860455303TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT58200.053.64307842
TCGTATG65850.051.759443
ACGTCTG87350.051.7378915
TATGCCG67000.051.48886546
GTCACAC77000.050.13431529
CGTATGC70400.049.05118644
CACGTCT95550.047.58630814
ACACGTC98950.046.29919413
AGTCACA85850.045.89053328
CAGTCAC88250.045.38509827
GTATGCC76550.045.29069545
CACACTT86600.043.77998431
CACACGT107150.042.6592612
TCACACT90450.042.48874730
GTCTGAA107650.042.014417
ATGCCGT84100.040.5684547
CCAGTCA104150.040.0132126
TCCAGTC118700.038.2475825
CGTCTGA121000.037.57797616
GCCGTCT89500.037.0808249
Produced by FastQC (version 0.11.2)