FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4s-43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4s-43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15386553
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG240330.15619482804238222TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG77600.046.45917515
CTCGTAT43350.045.99633442
TCGTATG49600.045.69494643
TATGCCG50750.045.61158446
GTCACAC58400.042.76991729
CACGTCT85950.041.98514614
ACACGTC87800.041.21743413
AGTCACA64200.040.14180428
CAGTCAC67400.039.82220527
GTATGCC58750.039.3415445
CACAGTG62550.038.27711533
CACACGT96300.037.47138612
ACACAGT64200.037.40046732
GTCTGAA99400.036.37430217
CGTATGC64650.035.3776944
CACACAG71050.034.6203231
CCAGTCA87800.033.98801426
ATGCCGT68900.033.646347
TCCAGTC106750.032.99595325
TCTGAAC113700.032.07250618
Produced by FastQC (version 0.11.2)