FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33646824
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14364254.269125074033734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT66100.08.1184741
TCGTGTA122650.07.41168714-15
CGGGAAT46100.07.351951
CCGGGAT61000.07.1766891
CGGAGAT66300.07.09998561
ATCGGAA277300.07.05387452
GATCGGA266500.06.88871771
GAGCGTC264600.06.8120849
CGGGCAT64700.06.76627541
TCGGAAG281050.06.70319753
CTCGGAT52550.06.62872461
GGGGAAT100850.06.5813381
CGGAAGA294900.06.43168834
CTGGAAT172500.06.16723251
GGATCGA36100.06.15808964
CGGGAAC59100.06.13301851
AGAGCGT279650.06.06959878
AGATCGG275900.06.02852794-95
AGCGTCG160000.06.01998910-11
CCCGGAT61000.06.01915841
Produced by FastQC (version 0.11.5)