FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34261336
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT102650.08.8313671
CCGGGAT99900.08.0292451
CGACGAT41200.07.948871
GTCGGTT57050.07.82034061
CCGGAAT88200.07.8028431
CGGGAAT75900.07.69160271
GATCGGA521100.07.554994694-95
GCGTCGT222900.07.497908694-95
CTCGGAT120750.07.35034941
AGATCGG587850.07.28353394-95
CGGCAAT59000.07.2400911
AGCGTCG259800.07.229077394-95
CCCACAT269750.07.14361571
CGCGGAT40550.07.13990551
CGGCGAT39550.07.080421
CGGGCAT103950.07.07718751
CGCCAAT59200.07.0552831
ATCGGAA509500.07.03642594-95
CTGGGAT374750.07.0165171
GGGGAAT169950.07.0098071
Produced by FastQC (version 0.11.5)