FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34081467
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT108750.08.72871
CGGGAAT76450.08.0086821
CCGGGAT100850.07.7652761
GGGGAAT168200.07.7034541
GTCGGAT53950.07.38986831
CGCATAT25900.07.3300861
GTCGGTT60500.07.2174081
GATCGGA498650.07.146789694-95
ATCGGAA493150.07.05294594-95
AGATCGG567200.06.84471294-95
CGGGGTT102900.06.82645461
CGGCGAT42850.06.7565941
CTCGGAT120550.06.7325211
CGCGGAT37850.06.64597651
CCGGAAT92150.06.6442081
AGCGTCG264300.06.611429794-95
CGTCGTG214900.06.57135994-95
CTGGGAT374200.06.5701431
GCGTCGT229500.06.546958494-95
CCCGGAT111300.06.5244681
Produced by FastQC (version 0.11.5)