FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34014242
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT80800.09.6334441
CGGGGAT92950.09.5996971
GGGGAAT170750.08.1999341
CGGGTAT40600.07.94935561
CCGGAAT92350.07.8632781
CGCGGAT34400.07.7264261
CCGGGAT94550.07.6301161
CGCCGAT52400.07.51788851
CGGAAAT80900.07.50949051
CCCACAT268150.07.4516661
ACATCTA215550.07.3741134
GTCGGTT60900.07.2479421
CGGCAAT54900.07.17554431
CGGGCAT95900.07.027791
AGATCGG567600.07.011677394-95
CATCTAC235800.07.00274475
GATCGGA507350.06.958692694-95
CTGGGAT378450.06.94784831
CCCGGAT101150.06.94456241
CGGGGTT98800.06.86954831