FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32980409
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA236900.010.7551844
ATCTACA254900.010.2017196
CATCTAC257200.010.0174825
CCCACAT282100.09.9095781
CGGGAAT73200.09.2718831
TACAGAC253950.08.652979
CGGGGAT94300.08.0528811
CCGGAAT79750.08.0342641
CACATCT347200.08.0078753
ACCTTAC103250.07.90498456
CCACATC364150.07.88332
GGGGAAT155700.07.834071
TCTACAG309700.07.83005247
CCGGGAT98950.07.67444751
CCGGTTT90300.07.46351771
ATCACCC176550.07.4177872
CTCGGAT103150.07.3619651
CGCCGAT56250.07.2563611
CGCGTAT16501.6916601E-107.19116161
CGGGTAT41100.07.15966751
Produced by FastQC (version 0.11.5)