FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34881204
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT85000.09.4367051
ACATCTA234350.08.624654
CGGGGAT109350.07.986411
CGGGCAT107650.07.98026131
ATCTACA254400.07.9085016
CCCACAT290000.07.88863041
CATCTAC259100.07.7645425
CGGCGAT44400.07.6966561
CGCGGAT40850.07.6683941
CCGGGAT101250.07.4065271
GCGGAAT53550.07.35649871
CCGGAAT95050.07.2404981
TACAGAC229500.07.03104939
CGACGAT42250.06.9649431
CGGAAAT85700.06.86741731
GTCGGAT51450.06.7342631
GATCGGA477200.06.720440494-95
GGGAAAT242950.06.72038361
CGGGTAT45300.06.7055491
CTGGGAT368900.06.65172771
Produced by FastQC (version 0.11.5)