FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34662281
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA36550.011.94673259
CGCGGGT64500.09.5666081
CGGGGTT115350.08.641241
CTACCGT53500.08.6048158
GTCGGAT38750.08.3293591
GGGGAAT176950.07.85943651
CCGGGAT94350.07.7976581
CGTATCG39350.07.7170343
CGGGGAT94200.07.70929861
TCGGATT60450.07.6925672
CTCGGAT100100.07.4919931
CGACCGT28200.07.40590141
CGCGGAT47600.07.17959451
ACTACCG62150.07.1778557
CGCGAAT24250.07.0463651
CGGGAAT77700.06.9029031
CGGGATT59350.06.877841
CCGGAAT78850.06.86242341
GATCGGA428050.06.78118694-95
GCGTCGT202600.06.75876994-95
Produced by FastQC (version 0.11.5)