FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36621997
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT105200.08.9783581
TACCGTA37300.08.6523069
CGGGGTT120450.07.95983741
CGCGGGT74550.07.89466431
CCCGTAT58300.07.7341871
CGACCGT28950.07.7056431
CGGCGAT65650.07.66356371
GGGGAAT190350.07.5552591
CGGGAAT84100.07.50611541
CGTATCG43300.07.45118333
CGAGAAT79500.07.28370481
CGCGGAT45950.07.23056361
CTCGGGT95100.07.0870821
GTCCGAT29950.06.9729331
CTCGGTT65500.06.95648051
CTCGGAT102400.06.95265871
CGGGTAT40350.06.82250831
ATCGGAA411050.06.80741394-95
CCGGGAT105950.06.7644981
GGGAAAT298150.06.74979641
Produced by FastQC (version 0.11.5)