FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32402164
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT94450.08.5932061
CCGGGAT95900.08.56226351
CGGGAAT71650.08.2142261
CGCGTAT10708.113784E-87.9845391
CGGGGTT112450.07.76639561
GGGGAAT159300.07.68714481
CGACCTA23850.07.55964664
GATCGGA479700.07.558083594-95
ATCGGAA458900.07.424029494-95
CCCGTAT37400.07.36067251
GCGTCGT205700.07.316122594-95
AGATCGG539650.07.08411294-95
CCGGAAT69100.07.00621131
CGCGGTT38700.06.990771
AGCGTCG233800.06.90457394-95
TCGGAAG460100.06.856938494-95
CGAGAAT70950.06.82352641
CGCGGGT68000.06.770551
CGTCGTG201150.06.725178294-95
CCCGGAT98700.06.68434331
Produced by FastQC (version 0.11.5)