FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32866183
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT108050.08.4776661
CGGGGAT96300.08.2306471
CGCGAAT18150.07.84491731
GCGTCGT229550.07.642930594-95
GGGGAAT160750.07.49939971
GATCGGA536800.07.46665294-95
AGATCGG599750.07.38850394-95
CGGGTAT36950.07.3215691
AGCGTCG256250.07.21766294-95
ATCGGAA510850.07.19443994-95
CGAGAAT74600.07.1256251
CGGGAAT72000.07.11926271
GGCGAAT50650.07.02789931
AGAGCGT317750.07.025234794-95
CGGGATT60450.06.98775151
CCGGGAT89800.06.976561
CGCGGAT39550.06.96025661
CTCGGAT83650.06.92209671
CGTCGTG225850.06.894488394-95
CGCGATT16551.3569661E-96.88267041
Produced by FastQC (version 0.11.5)