FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34600785
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT116650.08.8296221
CGCGAAT17400.08.4562841
GGGGAAT176700.08.1390411
CGGGTAT38700.08.0946871
CGGGAAT74550.07.95846841
CGGGGAT96150.07.7502821
GATCGGA540200.07.25715594-95
CCCGGAT99200.07.17706161
GCGTCGT235550.07.155886794-95
AGATCGG606650.07.10885194-95
ATCGGAA516000.07.086095394-95
AGCGTCG270650.07.053551794-95
CCGGGAT97700.07.04434351
GGGAAAT281450.06.93118761
CGGGCAT119400.06.87715631
CGACCGT25050.06.82121751
CGGGATT62800.06.80220941
GAATCGC74900.06.7781654
AGAATCG100400.06.7577943
CGAGAAT74750.06.60372921
Produced by FastQC (version 0.11.5)