FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34732726
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT39850.08.3374651
CCGGGAT104050.07.75481031
CCGGAAT76550.07.750491
CGGGAAT77150.07.69021461
AGATCGG587150.07.5760494-95
CGGGGAT106600.07.56930641
GATCGGA518500.07.54283694-95
CGACCTA22350.07.43033744
TCGGATT59750.07.385492
CGGGGTT120450.07.25062371
ATCGGAA501300.07.22303594-95
AGCGTCG256400.07.17695994-95
GGGGAAT179900.07.1763251
GTCGGAT45100.07.1564351
GCGTCGT223600.07.134577394-95
CGGCAAT63200.06.83421561
CGTCGTG219800.06.78199494-95
CGGGTAT39100.06.5551311
GTATCGA33300.06.55437954
CCGGTAT44200.06.5504621
Produced by FastQC (version 0.11.5)