FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31341526
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA33850.09.3938979
CGAAAAT139400.08.9546431
CTACCGT38550.08.3711778
CCGGGAT90650.07.8537811
CGTATCG26100.07.63515573
CTCGGAT75200.07.38453871
GATCGGA569600.07.145631394-95
CGGGATT69750.07.144971
AGATCGG631200.07.04338594-95
GGGGAAT160600.07.0337471
CGGGCTT97900.06.9812811
CGGGGTT131900.06.9809131
CGGGGAT89400.06.95487171
GCGTCGT252950.06.917512494-95
GTCGGAT34450.06.88868141
AGCGTCG276700.06.856469694-95
CGGGTAT32750.06.8114861
CGTCGTG251900.06.79533994-95
ATCGGAA540200.06.77315394-95
CGCAGAT102400.06.67448661
Produced by FastQC (version 0.11.5)