FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31251654
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAT146250.09.7361231
TACCGTA30850.09.0766779
CTACCGT37950.08.2534958
CCGGGAT92600.08.0985411
CGGGATT73200.08.0402811
CGCCCTT75700.07.83745051
CGGGGAT91700.07.71218731
CCGGAAT57100.07.48112731
GCCGTAT37350.07.377290760-61
GGGGAAT165700.07.30429551
CGCCGTA42150.07.26917558-59
GATCGGA552250.07.163203794-95
CTCGGAT71400.07.11289171
GCGTCGT244650.07.07417694-95
CGGGAAT67750.07.00569731
CGGGGTT137050.06.99572941
ATCGGAA529150.06.94127794-95
AGATCGG614850.06.909475394-95
AGCGTCG273900.06.804774394-95
CGTATCG27200.06.80303573
Produced by FastQC (version 0.11.5)