FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31141277
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAT125800.010.6401251
CGCCGTA41450.09.16829258-59
GCCGTAT40200.08.09524260-61
CCGGGAT90950.07.98485371
CGGGGTT134200.07.92271041
CGGGGAT119050.07.4956051
CGTATCA37900.07.45973462-63
GAATCGC98450.07.4219614
CCGTATC50850.07.380765460-61
CGGGAAT62550.07.28487631
GTCCGAT21000.07.23284151
AGAATCG119200.07.0454253
ATCGGAA603900.06.948389594-95
CGCGGGT61700.06.9236581
GATCGGA629100.06.817439694-95
CTGGAAT318100.06.8042371
GGGGAAT176400.06.7807891
GCGTCGT281300.06.744311394-95
AGATCGG687550.06.735799394-95
CGGGCTT92850.06.6968051
Produced by FastQC (version 0.11.5)