FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n02_ALm233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n02_ALm233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27561782
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA36500.011.973458-59
GCCGTAT37750.010.94849560-61
CGCGATT12300.09.6474221
CGTATCA35800.09.35702762-63
CCGTATC45500.08.71820660-61
TGGTCGC54950.08.688416554-55
CGAAAAT100700.08.6729081
CGGGGAT105450.08.2822361
CCGGGAT72950.07.61266851
TCGCCGT46650.07.586250358-59
TAGCGTT37350.07.380939586-87
CGAGAAT60650.07.35653731
CTCGTAT26550.07.329861
CGGGGTT110950.07.31551931
CGGGAAT55800.07.31544261
CTCGGTT61450.07.18352171
GTCGCCG68150.07.179781456-57
TAGATCG120150.07.063823794-95
AGCGTCG308550.07.019210394-95
ATCGGAA599700.06.97708594-95
Produced by FastQC (version 0.11.5)