FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33646824
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20111305.977176330223619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA516950.041.6656532
TCGGAAG526450.041.1758273
CGGAAGA529850.040.705744
AGAGCAC515600.040.697148
GATCGGA510500.040.556621
GAGCACA592250.035.50979
TCTCGCG158800.031.90566834-35
TCGCGCA147100.031.87285636-37
AAGAGCA712900.030.4188617
CGTCTGA348350.029.83921216-17
ACGTCTG349200.029.5903714-15
ACTCTCG172650.029.5840232-33
GAAGAGC740600.029.0786136
CACGTCT361500.028.87254714-15
ACACGTC357900.028.7519812-13
GCGATAG84150.028.70743434-35
CTCGCGC185050.028.41947434-35
CACACGT372800.028.01043912-13
TCTCGTA111100.027.65720242-43
CTCGTAT107650.026.92998344-45
Produced by FastQC (version 0.11.5)