FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34261336
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT892480.2604918850800214TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG171050.035.5723748-49
CGATAGA164150.035.34628336-37
GCGATAG176300.034.76957334-35
CTCGTAT165850.033.6238144-45
TCGTATG178000.033.53025444-45
AGCGATA187350.033.40339734-35
CGTATGC184100.033.3865346-47
TCTCGTA175950.030.02011742-43
ATGCCGT213400.028.0232248-49
ATCTCGT184100.027.55588242-43
TGCCGTC239050.025.51304850-51
GCCGTCT233050.025.12024550-51
CCGTCTT240150.024.83259652-53
GTATGCC245850.024.54682446-47
CAGCGAT257550.024.07738532-33
GATCTCG206550.024.0318940-41
ACAGCGA268350.023.45353332-33
GATAGAT257800.021.90736236-37
ATAGATC244300.021.34878538-39
TAGATCT247900.020.33938438-39
Produced by FastQC (version 0.11.5)