FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34081467
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1282120.37619272668045656TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG220750.038.04243548-49
CGATAGA212000.037.9993736-37
GCGATAG227750.037.2491934-35
TCGTATG227750.036.56080244-45
AGCGATA238450.036.25499334-35
CTCGTAT212850.036.19673544-45
CGTATGC236800.035.9955846-47
TCTCGTA224000.032.73060242-43
ATGCCGT259350.031.95004848-49
ATCTCGT229800.030.18887542-43
CCGTCTT284900.029.15143852-53
GCCGTCT285300.028.91071750-51
TGCCGTC296300.028.4786550-51
GTATGCC295850.028.2571646-47
CAGCGAT308900.027.67899332-33
ACAGCGA321800.027.14509832-33
GATCTCG256800.026.93153640-41
GATAGAT292250.026.63044436-37
ATCGGAA671100.025.771142
GATCGGA680600.025.1788521
Produced by FastQC (version 0.11.5)