FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34014242
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1236880.36363591462658496TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG215900.038.18228548-49
CGATAGA207750.037.66798836-37
GCGATAG221550.037.54106534-35
CTCGTAT206450.036.44494244-45
TCGTATG228700.036.0147144-45
AGCGATA232700.036.00761834-35
CGTATGC236300.035.2280446-47
TCTCGTA211300.033.8101642-43
ATGCCGT254550.032.09558548-49
ATCTCGT222200.030.58036842-43
TGCCGTC275500.029.74095750-51
CCGTCTT273350.029.3146352-53
GCCGTCT274500.029.1311950-51
GTATGCC288900.028.74008446-47
CAGCGAT297300.028.2075932-33
GATCTCG243650.027.7125740-41
ACAGCGA307050.027.33509832-33
GATAGAT298200.025.50185636-37
ATCGGAA679200.025.0788562
AGAGCAC687900.024.933838
Produced by FastQC (version 0.11.5)