FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32980409
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1165730.35346135337496876TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG212750.038.8256448-49
CGATAGA200600.038.70276336-37
GCGATAG221250.036.969634-35
CTCGTAT202100.036.75908744-45
CGTATGC228400.036.48776246-47
AGCGATA230600.036.44904334-35
TCGTATG224000.035.90072344-45
TCTCGTA213050.033.05308542-43
ATGCCGT258900.031.18929348-49
ATCTCGT217750.030.80174842-43
CCGTCTT268650.029.75689352-53
GCCGTCT266650.029.56128750-51
TGCCGTC281650.029.11685850-51
GTATGCC284400.028.61834346-47
CAGCGAT294500.028.0001932-33
GATCTCG236350.027.88529440-41
ATCGGAA603450.027.7707612
ACAGCGA304800.027.7007332-33
GATCGGA607750.027.3139231
GATAGAT281300.026.50205836-37
Produced by FastQC (version 0.11.5)