FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34881204
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1943040.5570449919102562TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG316350.041.00526448-49
CGATAGA304000.040.53861636-37
GCGATAG327850.039.76299334-35
CTCGTAT300650.039.38740544-45
TCGTATG325800.039.24825344-45
AGCGATA339000.039.12071234-35
CGTATGC341650.038.37240646-47
TCTCGTA306950.036.7146442-43
ATCGGAA752200.035.4644242
ATGCCGT364500.035.0086348-49
GATCGGA756950.034.9468771
ATCTCGT310750.034.5154542-43
AGAGCAC788600.033.7240528
CGGAAGA798200.033.592914
GAGCACA795500.033.2763379
CCGTCTT384900.033.04850852-53
TGCCGTC391100.033.04657750-51
TCGGAAG821600.032.7692643
GCCGTCT387250.032.5165250-51
GTATGCC396900.032.51022346-47
Produced by FastQC (version 0.11.5)