FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34662281
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1429650.4124512175064301TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC239250.040.14396746-47
TCGTATG235700.039.7715744-45
TATGCCG245150.038.82861748-49
CTCGTAT226150.038.59454744-45
AGCGATA267150.036.5472734-35
CGATAGA256600.035.66171636-37
GCGATAG271600.035.5637134-35
TCTCGTA241750.034.22802742-43
ATCTCGT238300.033.06911542-43
ATCGGAA626550.031.3789882
GTATGCC304500.031.12050846-47
TCGGAAG643650.030.8327053
CCGTCTT306650.030.6618952-53
GATCGGA636700.030.6156181
ATGCCGT309500.030.44090848-49
GCCGTCT304550.030.43641750-51
GAGCACA655300.030.1972279
CGGAAGA661050.029.9421834
AGAGCAC678750.029.153958
CAGCGAT336850.028.68893632-33
Produced by FastQC (version 0.11.5)