FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36621997
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1294430.3534569674067747TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG213850.039.0705544-45
CGTATGC220950.038.81453346-47
TATGCCG226550.037.4775748-49
CTCGTAT207050.037.1419444-45
AGCGATA243700.035.77619634-35
CGATAGA231850.035.2790136-37
GCGATAG245900.035.0118334-35
TCTCGTA218950.033.51819642-43
ATCTCGT221700.031.6240742-43
ATCGGAA597700.029.380822
ATGCCGT288200.029.20513248-49
CCGTCTT288700.028.98994352-53
GCCGTCT287200.028.83534450-51
TCGGAAG614400.028.8143
GATCGGA605100.028.744091
GTATGCC297700.028.4647246-47
GAGCACA629700.028.0833789
CAGCGAT312650.027.57502632-33
CGGAAGA640800.027.574984
AGAGCAC654650.027.0058128
Produced by FastQC (version 0.11.5)