FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32402164
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT146950.035.44270336-37
TCGCGCA166250.034.6423936-37
TCTCGCG179000.033.83360734-35
CGCGTAT24750.030.3232744-45
ACTCTCG201350.030.12527732-33
ATCGCGT33300.028.81396142-43
CTCGCGC224550.026.70603634-35
TCGCGTA28600.025.49400942-43
GCGCATC193250.024.72691538-39
CGTATGC57650.024.38846846-47
CTCTCGC253050.023.9892332-33
CGCATCG89400.023.6967538-39
GAGCACA564000.022.6210989
TATGCCG72600.022.01597448-49
AGAGCAC586050.021.6321668
TCGTATG53800.021.49863244-45
CTCGTAT59700.021.44265644-45
TCGGAAG606850.020.7970473
ATCGGAA597500.020.7490442
CGGAAGA615600.020.3703334
Produced by FastQC (version 0.11.5)