FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32866183
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT158850.035.89760236-37
TCGCGCA178300.035.1785236-37
TCTCGCG193350.034.30748734-35
CGCGTAT30900.030.66732644-45
ACTCTCG224000.029.94193332-33
ATCGCGT38900.029.91644542-43
TCGCGTA32200.028.91303642-43
CTCGCGC233600.027.93869434-35
GCGCATC206000.025.55996738-39
CGTATGC63350.025.30564146-47
CTCTCGC266350.024.91362232-33
TATGCCG75700.024.12616748-49
CGCATCG97200.024.06754538-39
TCGTATG56050.023.34734344-45
GAGCACA610800.022.5910269
CTCGTAT67500.022.2369344-45
AGAGCAC638750.021.542988
GTCACTC324600.020.85974128-29
TCGGAAG672600.020.628653
CGGAAGA676750.020.4529574
Produced by FastQC (version 0.11.5)