FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34600785
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT176300.036.8167636-37
TCGCGCA196850.036.31527736-37
TCTCGCG216000.035.0202334-35
CGCGTAT31500.031.66682844-45
ACTCTCG239650.031.59419632-33
ATCGCGT42650.029.34680742-43
CTCGCGC259200.028.78036334-35
TCGCGTA34800.028.18643242-43
GCGCATC219800.027.52118138-39
CGTATGC68700.026.7919846-47
CTCTCGC290200.026.04987732-33
CGCATCG104750.025.9380838-39
TATGCCG83900.025.22166348-49
GAGCACA642100.024.3008049
CTCGTAT73950.023.99099544-45
TCGTATG64200.023.49116144-45
AGAGCAC665350.023.4659168
TCGGAAG692150.022.6675133
CGGAAGA692950.022.6001824
ATCGGAA688300.022.4908182
Produced by FastQC (version 0.11.5)