FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34732726
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT163150.036.3051936-37
TCGCGCA184200.035.83094836-37
TCTCGCG204600.034.25521534-35
CGCGTAT29400.031.34363744-45
ACTCTCG226700.031.02074232-33
ATCGCGT38300.028.52495642-43
CTCGCGC245550.027.84612734-35
GCGCATC213600.026.3406438-39
CGTATGC63850.025.85157646-47
TCGCGTA35000.025.51442342-43
CTCTCGC282600.024.77539432-33
CGCATCG100400.024.31766738-39
TATGCCG75650.024.14219748-49
GAGCACA620250.022.8747779
CTCGTAT69500.022.62237244-45
AGAGCAC635650.022.357958
TCGTATG60250.022.15359944-45
ATCGGAA654400.021.5946352
TCGGAAG665600.021.5380123
CGGAAGA665150.021.4096934
Produced by FastQC (version 0.11.5)