FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31341526
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT693190.22117302137745304TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT408020.13018510968483155TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA871900.045.015269
AGAGCAC902700.043.731928
CGCGCAT389200.042.4897636-37
TCGCGCA432350.042.19325336-37
TCTCGCG466500.041.4570534-35
CGCGTAT62000.040.9495244-45
ATCGGAA965800.040.747622
CGGAAGA978150.040.470654
TCGGAAG993050.040.0691573
ACTCTCG486100.039.78552632-33
GATCGGA991000.039.5404171
ATCGCGT78150.038.7478142-43
CTCGCGC498650.038.1840234-35
AAGAGCA1039800.038.1210177
GCGCATC403150.037.62655638-39
TATGCCG134800.037.26305448-49
TCGCGTA65250.036.9082442-43
CTCTCGC532550.036.13250732-33
GAAGAGC1105100.036.079426
CGCATCG185150.035.9810138-39
Produced by FastQC (version 0.11.5)