FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31251654
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT864630.2766669565713226TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT508240.16262819241503185TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA940350.048.794589
AGAGCAC963450.047.797228
ATCGGAA1027100.045.0821722
CGGAAGA1038150.044.7528844
TCGGAAG1050100.044.5333183
GATCGGA1049700.043.853341
CGCGCAT453400.043.00522236-37
TCGCGCA502200.042.8129736-37
TCTCGCG541950.041.91278534-35
AAGAGCA1110750.041.749677
CGCGTAT73700.040.82951744-45
ACTCTCG558400.040.80988332-33
GAAGAGC1163350.040.014026
ATCGCGT93400.039.71953242-43
TCGCGTA74150.039.1408342-43
CTCGCGC570950.039.0393334-35
GCGCATC465300.038.87410438-39
CGTATGC141000.037.814346-47
TATGCCG159600.037.81187448-49
CGCATCG212600.037.624938-39
Produced by FastQC (version 0.11.5)