FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31141277
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1001000.3214383276575331TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT585210.18792100272573922TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1068150.051.161459
AGAGCAC1111150.049.5107358
CGGAAGA1203100.046.208934
ATCGGAA1199150.046.05242
TCGGAAG1235350.045.260153
GATCGGA1223950.044.9029051
TCGCGCA589600.043.32642436-37
CGCGCAT533750.043.18343436-37
AAGAGCA1287800.042.9480677
CGCGTAT85000.042.3310344-45
TCTCGCG643400.042.1807734-35
ATCGCGT106700.041.4906342-43
GAAGAGC1357400.041.0507666
ACTCTCG665000.040.8608232-33
GCGCATC525950.040.3518538-39
CTCGCGC662500.039.9322434-35
TATGCCG175200.039.7996348-49
CGTATGC157800.039.1468146-47
TCGCGTA89500.038.7434642-43
CTCGTAT149750.038.17458744-45
Produced by FastQC (version 0.11.5)