FastQCFastQC Report
Fri 17 May 2019
HFMJ3BGXB_n01_ALm233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFMJ3BGXB_n01_ALm233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27561782
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1299820.4716023078623871TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT769350.27913652317546084TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGGAT275640.1000080473751661TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1214700.058.1786049
AGAGCAC1254550.056.644858
CGGAAGA1356750.052.7636384
ATCGGAA1364700.052.404532
TCGGAAG1388850.051.869233
GATCGGA1390700.051.032321
AAGAGCA1419600.050.2766047
GAAGAGC1486150.048.236516
GGAAGAG1626800.044.2559435
TCGCGCA750550.044.22463236-37
CGCGCAT681100.044.11733236-37
TCTCGCG802200.043.79604734-35
CGCGTAT108400.043.33722344-45
ATCGCGT132700.042.79315642-43
ACTCTCG822300.042.7631832-33
GCGCATC661050.041.9885138-39
TATGCCG221150.041.721848-49
CTCGCGC821450.041.7173234-35
TCGCGTA109000.041.53001442-43
CGTATGC204300.040.5829146-47
Produced by FastQC (version 0.11.5)