FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36179879
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG997589827.573055177989954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT41650.011.6426132
CGTATCA20300.09.00656250-51
GCCGTAT20700.08.94589948-49
CCGTATC21650.08.66301248-49
CGCCGTA21450.08.41105646-47
TTCCCTA25350.08.2353988
GGAGAAT61850.07.78916551
TCGCCGT24450.07.67030246-47
GTGCCTA28600.07.635270620-21
CCGATCT16905.456968E-127.5952552
TCCGATC14808.58563E-107.4126821
CGGGGAT22500.07.20784851
CGGTTCG8057.4760464E-107.074458612-13
CTGAAAT58750.06.73875241
GGGGAAT34550.06.62677861
TCCCTAC27450.06.5688459
CTACCGT8700.00127976426.5444318
CCGGTAT8750.00128609456.54157731
CGGGATT13352.9590701E-66.43132541
CGGGTCT24400.06.4188323
Produced by FastQC (version 0.11.5)