FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16043662
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA196500.013.5016622
AAGGGGG177450.012.30809980-81
CGCGTAT3207.097524E-411.865651
AGAGCGG58050.010.5485938
AAAAGGG220300.010.46413478-79
GGGGGAG215700.010.2317273
GGGGAGA174500.09.41109854
GAGCGGG91700.09.266669
TAGGGCG18850.08.93912332-33
CGGGGAT50200.08.8874191
GTCGATT12301.8189894E-118.8751191
GAGAGCG68300.08.6873147
CGCCGTA17350.08.62624558-59
ACGATCG17650.08.6050093
CGGGAAT31050.08.5600821
CTCGGAT38500.08.5062841
TAGGGGG56150.08.16292950-51
GCGTCGT142400.08.16017694-95
CGGGGTT72750.08.1550861
CCGGGAT42550.07.91972881
Produced by FastQC (version 0.11.5)