FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15816225
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA134850.012.3526042
TACCGTA15700.012.0951049
CGCGGGT30200.011.0000891
CGACCGT12450.010.2919931
CTACCGT19250.010.1105868
CGGGGAT48600.09.3743261
AAGGGGG132700.09.29499380-81
CGTATCG18250.08.3214253
CGCGATT5250.00455308758.1355761
CGGGTAT17600.08.0893511
CCCGTAT24350.07.9907951
CGGGAAT36300.07.9749561
CGCTAAT7753.9474085E-57.96061751
CCGGGAT43600.07.94587041
GGGGGAG178100.07.8341883
CGGGGTT48950.07.75608251
TAGGGGG51500.07.747050350-51
CGACCTA9205.0499693E-67.73812964
CTCGGTT28250.07.72759681
GCGTCGT138150.07.65440794-95
Produced by FastQC (version 0.11.5)