FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15813535
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16450.012.1204019
CTACCGT20600.010.8302418
GGGGGGA146600.010.4563452
CGCGATT5203.7366655E-510.0391411
CGGGGAT50250.09.82210351
CGCGGGT33000.09.7791741
AAGGGGG143150.08.948480-81
CTCGGTT30150.08.4998971
CGCGCAT10801.0755684E-88.34905051
GTCCGAT15450.08.2935881
CGGGGTT52950.08.2457291
CCGGAAT37150.07.79252531
AAAAGGG173450.07.71368978-79
GCGTCGT134000.07.66106494-95
ACTACCG28000.07.62873137
GATCGGA306700.07.616401794-95
CGGGCAT65850.07.5672961
CGGGAAT37050.07.4292851
CGCGTGT23700.07.40901661
AGCGTCG156300.07.373825694-95
Produced by FastQC (version 0.11.5)