FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16220064
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16850.014.6503029
GGGGGGA160600.011.1701622
CTACCGT21800.011.1052258
CGGGGAT52250.011.0817461
CGCGGGT31000.010.8700441
AAGGGGG149150.09.76755280-81
CTCGTAT15950.09.2243471
CGGGAAT35450.08.5683731
AAAAGGG183100.08.15102978-79
CGGGGTT52100.08.1074991
GGGGAAT90500.08.0761951
CCGTATG21100.07.87484710-11
CCGGGAT46600.07.74038031
ACCGTAT18750.07.7224510-11
CGCGATT5650.0087896257.56012061
CGACCTA9507.951503E-67.49396564
GCGTCGT141600.07.48463394-95
ACTACCG29750.07.3395567
CGAGGAT47850.07.2406171
CCGGAAT36150.07.2208621
Produced by FastQC (version 0.11.5)