FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15832015
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA15700.013.0019969
CTACCGT20950.012.0084358
CGCGGGT31550.011.8836811
CGTATCG16400.010.7072273
CGGGGAT49900.010.4620161
GGGGGGA143750.010.0363052
AAGGGGG136800.08.99931880-81
GTATCGA21150.08.9761634
GTCCGAT12955.456968E-128.7955851
CGACCTA8652.2348104E-78.7789984
CGGCGAT32300.08.6690751
CTCGGTT28250.08.5679111
CCCGTAT23200.08.387241
ACTACCG26850.08.3087527
GGGGAAT87100.07.73737051
AAAAGGG170650.07.72915678-79
TAGATCG50100.07.683992494-95
CGACCGT11352.1889718E-77.52661851
GGCGAAT20950.07.4757231
CGGGTCT60600.07.4399523
Produced by FastQC (version 0.11.5)