FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15709930
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA18100.013.11389
AAGGGGG159350.011.08152480-81
CGCGGGT30250.010.5124751
CGACCGT11850.010.4138321
GGGGGGA173850.010.31924252
AAAAGGG180150.09.99967678-79
CGGGGAT49950.09.3120691
CTACCGT24550.09.2811848
AAAGGGG163500.08.72136578-79
CCCGTAT23300.08.5555711
CGTATCG18000.08.4376593
TAGGGGG54700.08.03176150-51
CGTATGG16050.07.986547512-13
ACTACCG29550.07.71056757
CCGCAAT21800.07.62021261
GGGGAAT84750.07.56048441
CGGGGTT52250.07.5395841
CCGTATC29000.07.5281892
GTCGGTA12702.377783E-87.47449541
AGGGGGG194750.07.45635380-81
Produced by FastQC (version 0.11.5)