FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16788775
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA17800.015.4680799
CTACCGT22700.012.9647988
GGGGGGA168700.011.9842712
AAGGGGG158500.010.72093280-81
CGCGATT6051.7157781E-610.1983141
CGCGGGT30750.09.2607571
CGTATCG19500.09.2485823
CGGGGAT51850.09.2451311
AAAAGGG194350.08.81635378-79
ACTACCG33200.08.5782017
CGGGGTT52650.08.4736381
CGGGAAT40200.08.1463571
TAGGGGG56450.08.11977850-51
CGACCGT14053.8198777E-118.1072831
CCGGGAT45150.08.094191
GGGGAAT92250.08.0774381
CGGGTAT17100.08.0490061
CGCGGAT21350.08.0028561
GGGGGAG212250.07.8708413
GCGTCGT151800.07.74908394-95
Produced by FastQC (version 0.11.5)