FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15027809
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3000.005918693311.0734881
TACCGTA14950.010.79606159
CGGGGAT50200.09.0755971
CTACCGT20050.08.7598418
CGCGGAT18450.08.7456131
CGCGATT5659.42526E-48.3996111
TCGCATA9155.2715586E-78.2983022
GGGGGGA130800.08.2721322
CGGGGTT49400.07.78154331
CGATCGT6100.00200780887.7804094
GATCGGA265400.07.77213194-95
CTCGTAT13651.4479156E-97.64887761
GCGTCGT111750.07.61301794-95
GGGGAAT83650.07.6023271
AGATCGG295800.07.543774694-95
CGGGAAT36250.07.46233131
CTCGGAT40750.07.4534961
AAGGGGG125900.07.4191180-81
GGGGGAT76700.07.36307571
GTAATCG11051.1482371E-67.3012253
Produced by FastQC (version 0.11.5)