FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16082616
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA145350.09.6649572
CCGGGAT48700.09.160991
CGCGGGT36350.09.0092411
CGCGAAT8551.8704668E-78.8817261
CGCGATT5557.887712E-48.5516621
CGGGGAT60100.08.0550681
GCGTCGT143650.07.94017894-95
AAGGGGG133750.07.871398480-81
CGCGTAA7251.5788338E-47.8557341
GTCGGAT18300.07.78061061
CCCGTAT24550.07.53974441
ACGTGCG29150.07.49037086
TGCGCGA19950.07.376689
CTCGGAT46550.07.34101871
AGCGTCG170550.07.328728794-95
CGTCGTG139750.07.328584794-95
CGGGGTT56500.07.30826571
GTCGAAT15101.4515535E-97.22926951
CGGGAAC49250.07.2276741
CCGGAAT38100.07.22514441
Produced by FastQC (version 0.11.5)